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JoVE Journal
Biochemistry

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Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore
 

Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore

Article DOI: 10.3791/60659-v 09:15 min January 21st, 2020
January 21st, 2020

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Summary

A protocol for the induction of eryptosis, programmed cell death in erythrocytes, using the calcium ionophore, ionomycin, is provided. Successful eryptosis is evaluated by monitoring the localization phosphatidylserine in the membrane outer leaflet. Factors affecting the success of the protocol have been examined and optimal conditions provided.

Tags

Keywords: Eryptosis Red Blood Cells Calcium Ionophore Ionomycin Ringer Solution Glucose-free Buffer Hematocrit Cell Suspension Centrifugation Incubation
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