/

Cancer Research

/

结合使用尾静脉转移分析与实时 Vivo 成像，以量化乳腺癌转移殖民化和肺负担

JoVE Journal
Cancer Research

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Cancer Research

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Please note that all translations are automatically generated. Click here for the English version.

结合使用尾静脉转移分析与实时 Vivo 成像，以量化乳腺癌转移殖民化和肺负担

DOI:

10.3791/60687-v

•

10:32 min

•

December 19, 2019

•

Janine S. A. Warren, Paul J. Feustel, John M. Lamar

¹Department of Molecular and Cellular Physiology,Albany Medical College, ²Department of Neuroscience and Experimental Therapeutics,Albany Medical College

Chapters

00:04Title
00:32Generation of Cancer Cells Stably Expressing Luciferase and a Fluorescent Protein
01:59Tail Vein Injection of Labeled Cancer Cells
04:17Monitoring the Metastatic Burden by Bioluminescence with an in vivo Live Animal Imaging Device
06:18Quantification of the Number and Size of Metastases and Analysis of the Imaging Data
08:01Results: YAP and TAZ are Required for Metastatic Colonization and Subsequent Growth of Breast Cancer Cells in Syngeneic Mice
09:36Conclusion

Summary

Automatic Translation

所述方法将实验性尾静脉转移测定与活体动物成像相结合，除了对肺部转移数和大小进行定量外，还能够实时监测乳腺癌转移的形成和生长。

Tags

Keywords: Tail Vein Metastasis Assay Real-time In Vivo Imaging Breast Cancer Metastatic Colonization Metastasis Burden Lung T1 Cells Luciferase Fluorescent Protein Viral Transduction Cell Culture Cell Counting Cell Injection Animal Model

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

内髂动脉注射显微骨转移的效率和选择性建模

共培养化验，以研究胶质母细胞瘤入侵的巨噬细胞和小胶质细胞刺激

肝大肠癌转移的高级动物模型：成像技术和转移性克隆性质

门静脉注射模型来研究肝转移乳腺癌

应用肺转移法研究骨肉瘤转移性肺定植的实用思考

体内在肿瘤微环境中 pH 值、 pO₂、氧化还原状态、磷酸盐和谷胱甘肽浓度的 EPR 评价

循环肿瘤细胞的微操作, 用于下游分子分析和转移电位评估

肿瘤细胞外侧尾静脉注射后肺转移的病理分析

利用计算机图像分析提高4T1乳腺癌模型中肺转移的定量

通过炎症性乳腺癌细胞的尾静脉注射模拟脑转移

Read Article