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Immunology and Infection

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微生物通过微血管内皮细胞对血脑屏障渗透性分析

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Analyzing the Permeability of the Blood-Brain Barrier by Microbial Traversal through Microvascular Endothelial Cells

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微生物通过微血管内皮细胞对血脑屏障渗透性分析

DOI:

10.3791/60692-v

•

06:26 min

•

February 14, 2020

•

Veronika Weber, Kaya Bork, Rüdiger Horstkorte, Heidi Olzscha

¹Institute of Physiological Chemistry,Martin Luther University Halle-Wittenberg

Chapters

00:05Introduction
00:39Growth of Blood-Brain Barrier Mimicking Cells
02:58Preparation of Bacteria and Cell Treatment
03:40Measurement of Permeability
04:46Results: Effects of Protein Glycation on the Blood-Brain Barrier
05:48Conclusion

Summary

Automatic Translation

人类血脑屏障选择性地防止亲水分子和病原体渗透到大脑中。几种疾病，包括脑膜炎和术后精神错乱，与血脑屏障的渗透性增加有关。在这里，我们描述了一个内皮细胞培养模型，以测试通过微生物遍历的屏障渗透性。

Tags

Blood-brain Barrier Microbial Traversal Microvascular Endothelial Cells Bacterial Meningitis Postoperative Delirium High Throughput Analysis Collagen IV Fibronectin Human Microvascular Endothelial Cells Trypsin EDTA FCS Trypan Blue E.coli

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