Time-lapse Imaging of Mouse Macrophage Chemotaxis

Journal

Immunology and Infection

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Time-lapse Imaging of Mouse Macrophage Chemotaxis

DOI:

10.3791/60750-v

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09:33 min

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April 02, 2020

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Esther van den Bos, Stefan Walbaum, Markus Horsthemke, Anne C. Bachg, Peter J. Hanley

¹Institut für Molekulare Zellbiologie

Chapters

00:04Introduction
01:04Prefilling of Chemotaxis Slides
02:25Isolation of Mouse Resident Peritoneal Macrophages
03:40Seeding Peritoneal Cells and Adding Chemoattractant
07:31Results: Chemotaxis of Mouse Macrophages
09:07Conclusion

Summary

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Here we describe methods using time-lapse, phase-contrast microscopy to image mouse resident peritoneal macrophages in a chemotactic complement C5a gradient. The protocols can be extended to other immune cells.

Time-lapse Imaging of Mouse Macrophage Chemotaxis

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