Journal
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小鼠巨噬体化学术的延时成像
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Time-lapse Imaging of Mouse Macrophage Chemotaxis
DOI:

09:33 min

April 02, 2020

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Chapters

  • 00:04Introduction
  • 01:04Prefilling of Chemotaxis Slides
  • 02:25Isolation of Mouse Resident Peritoneal Macrophages
  • 03:40Seeding Peritoneal Cells and Adding Chemoattractant
  • 07:31Results: Chemotaxis of Mouse Macrophages
  • 09:07Conclusion

Summary

Automatic Translation

在这里,我们描述了使用延时,相位对比显微镜成像小鼠常驻腹腔巨噬细胞在化学策略补充C5a梯度的方法。协议可以扩展到其他免疫细胞。

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