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Time-lapse Imaging af Mus Makrofag Chemotaxis
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Time-lapse Imaging of Mouse Macrophage Chemotaxis
DOI:

09:33 min

April 02, 2020

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Chapters

  • 00:04Introduction
  • 01:04Prefilling of Chemotaxis Slides
  • 02:25Isolation of Mouse Resident Peritoneal Macrophages
  • 03:40Seeding Peritoneal Cells and Adding Chemoattractant
  • 07:31Results: Chemotaxis of Mouse Macrophages
  • 09:07Conclusion

Summary

Automatic Translation

Her beskriver vi metoder ved hjælp af time-lapse, fase-kontrast mikroskopi til billedmus hjemmehørende peritoneal makrofager i en kemotaktisk supplement C5a gradient. Protokollerne kan udvides til andre immunceller.

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