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Time-lapse Imaging av mus makrofag Chemotaxis
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Time-lapse Imaging of Mouse Macrophage Chemotaxis
DOI:

09:33 min

April 02, 2020

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Chapters

  • 00:04Introduction
  • 01:04Prefilling of Chemotaxis Slides
  • 02:25Isolation of Mouse Resident Peritoneal Macrophages
  • 03:40Seeding Peritoneal Cells and Adding Chemoattractant
  • 07:31Results: Chemotaxis of Mouse Macrophages
  • 09:07Conclusion

Summary

Automatic Translation

Här beskriver vi metoder med time-lapse, fas-kontrast mikroskopi till bild mus bosatt peritoneal makrofager i ett chemotactic komplement C5a gradient. Protokollen kan utvidgas till andra immunceller.

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