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Identificazione delle trappole extracellulari neutrophil in Paraffin-Embedded Feline Arterial Thrombi utilizzando la microscopia immunofluorescenza
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1Veterinary Medical Teaching Hospital, School of Veterinary Medicine,University of California, Davis, 2Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine,University of California, Davis, 3Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine,University of California, Davis
Chapters
- 00:04Introduction
- 01:15Deparaffinization, Rehydration, and Heat-Induced Antigen Retrieval
- 02:29Immunolabeling and Autofluorescence Quenching
- 05:13Neutrophil Extracellular Trap Identification
- 06:08Results: Phase Contrast and Immunofluorescence Images
- 07:20Conclusion
Descriviamo un metodo per identificare le trappole extracellulari neutrofiche (NET) nei trombi arteriosi cardiogenici felini fissi e incorporati in parina di formaldeide utilizzando il recupero di antigeni indotti dal calore e un doppio protocollo di immunoetichettatura.
