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Journal
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Genetics
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外子跳过直接重新编程的线管从人类尿衍生细胞获得
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal
Genetics
Exon Skipping in Directly Reprogrammed Myotubes Obtained from Human Urine-Derived Cells
Please note that all translations are automatically generated.
Click here for the English version.
外子跳过直接重新编程的线管从人类尿衍生细胞获得
DOI:
10.3791/60840-v
•
06:20 min
•
May 07, 2020
•
Hotake Takizawa
,
Mitsuto Sato
,
Yoshitsugu Aoki
1
Department of Molecular Therapy, National Institute of Neuroscience
,
National Center of Neurology and Psychiatry
Chapters
00:04
Introduction
00:54
Isolation and Primary Culture of Urine-Derived Cells
02:31
Infection with
MYOD1
-Retroviral Vector
03:20
Myogenic Differentiation of
MYOD1
-Transduced UDCs
03:53
Evaluation of Exon Skipping Efficiency by RT-PCR
05:05
Results: Restoration of Dystrophin mRNA in UDCs
05:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在本文中,我们描述了一个详细的协议,使用
MYOD1
- 转化的尿液衍生细胞有效建模Duchenne肌肉营养不良肌,以评估脱外器跳过后肌营养不良的mRNA和蛋白质水平的恢复。
Tags
Exon Skipping
Direct Reprogramming
Human Urine-derived Cells
Myotubes
Duchenne Muscular Dystrophy
3-deazaneplanocin A Hydrochloride
DZnep
Histone Methyltransferase Inhibitor
Autologous
Precision Medicine
Muscular Diseases
Centrifugation
Cell Culture
Gelatin-coated
Retrovirus
MYOD1
Puromycin
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