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1Research and Innovation,Murdoch University, 2Centre for Digital Agriculture,Curtin University, 3Centre for Integrative Metabolomics and Computational Biology, School of Science,Edith Cowan University, 4Centre for Computational and System Medicine, Health Futures Institute,Murdoch University, 5Department of Primary Industries and Regional Development, 6State Agricultural Biotechnology Centre,Murdoch University
Chapters
- 00:05Introduction
- 01:04Preparation of Grains
- 01:46Metabolite Extraction
- 03:31Preparing Samples for LC-MS Analysis
- 04:10Setting Up Liquid Chromatography Mass Spectrometry
- 05:13Data Processing
- 05:38Results: Biologically Relevant Features Differ Across Wheat Varieties
- 06:42Conclusion
Apresenta-se um método para a análise não direcionada de metabólitos e lipídios de grãos de trigo. O protocolo inclui um método de extração metabólica acetonitrila e metodologia de espectrometria de cromatografia líquida de fase invertida, com aquisição em modos positivos e negativos de ionização eletrospray.
