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Stimulering af stamcellenicher og vævsregenerering i musehud ved omskiftelig protoporphyrin IX-afhængig fotogenerering af reaktive iltarter in situ
JoVE Journal
Biology
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JoVE Journal Biology
Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ
DOI:

10:05 min

May 08, 2020

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Chapters

  • 00:04Introduction
  • 01:26Hair Growth and Burn Induction and Long‐Term 5‐bromo‐2 -deoxyuridine (BrdU) Label Retaining Cell (LRC) Generation
  • 02:58Transient Nonlethal Reactive Oxygen Species (ROS) Production
  • 04:37BrdU Detection in Mouse Tail Skin
  • 05:38ROS Detection in Mouse Tail Skin
  • 07:16Results: Effects of Photodynamic mALA Treatment and Transient ROS Production
  • 09:00Conclusion

Summary

Automatic Translation

Formålet med denne protokol er at fremkalde forbigående in vivo-produktion af ikke-dødelige niveauer af reaktive iltarter (ROS) i musehud, hvilket yderligere fremmer fysiologiske reaktioner i vævet.

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