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Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes
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Chapters
- 00:05Introduction
- 01:06Equipment Setup
- 02:46Lysolipid-Containing Temperature-Sensitive Liposome (LTSL) Preparation
- 04:48Remote Doxorubicin (DOX) Loading
- 05:48Laser Heating and Triggered Release
- 07:13Results: Representative LTSL10 Characterization, Loading, and Photothermal Heat Triggering
- 08:59Conclusion
The protocol presents the optimized parameters for preparing thermosensitive liposomes using the staggered herringbone micromixer microfluidics device. This also allows co-encapsulation of doxorubicin and indocyanine green into the liposomes and the photothermal-triggered release of doxorubicin for controlled/triggered drug release.
