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Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution
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Bioengineering
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JoVE Journal Bioengineering
Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution

Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution

DOI:
10.3791/60920-v

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06:29 min

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May 02, 2020

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, , , , , , , , ,
1Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital,Fudan University, 2Shanghai Institute of Cardiovascular Diseases, 3Center for Medical Research and Innovation, Shanghai Pudong Hospital,Fudan University Pudong Medical Center, 4Institutes of Biomedical Sciences and Department of Systems Biology for Medicine, Shanghai Medical College,Fudan University

Chapters

  • 00:04Introduction
  • 01:05Cell-Laden Silk Fibroin-Gelatin Methacryloyl (SF-GelMA) Preparation
  • 03:28SF-MultiLayered-GelMA Loading, Reheating and Bioprinting
  • 05:16Results: Representative Bioprinting Results Using Three Different Cell-Laden Bioinks
  • 05:59Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we developed a novel multilayered modified strategy for liquid-like bioinks (gelatin methacryloyl with low viscosity) to prevent the sedimentation of encapsulated cells.

Tags

Multilayered Hydrogel BioinkThree-dimensional BioprintingHomogeneous Cell DistributionGelatin Methacrylate (GelMA)Silk FibroinBioink PreparationCell EncapsulationCell SedimentationExtrusion PrintingDrug ScreeningRegeneration Medicine

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