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激光捕获胶质瘤分区域的微切，用于肿瘤内异质性、肿瘤流和入侵的空间和分子表征
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Neuroscience
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JoVE Journal Neuroscience
Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion
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激光捕获胶质瘤分区域的微切，用于肿瘤内异质性、肿瘤流和入侵的空间和分子表征

DOI:
10.3791/60939-v

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09:09 min

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April 12, 2020

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, , , , , ,
1Dept. of Neurosurgery,University of Michigan Medical School, 2Dept. of Cell and Developmental Biology,University of Michigan Medical School, 3Dept. of Ophthalmology & Visual Science,University of Michigan Medical School, 4Rogel Cancer Center,University of Michigan Medical School

Chapters

  • 00:05Introduction
  • 01:17Animal Perfusion and Brain Preservation
  • 02:01Glioma Brain Tumors Tissue Cryopreservation
  • 02:52Frozen Brain Tumor Tissue Sectioning
  • 03:58Cryopreserved Brain Tissue Section Fixation and Staining
  • 04:48Laser Capture Microdissection
  • 06:30Results: Representative RNA Quality Control and Library Preparation
  • 07:55Conclusion

Summary

Automatic Translation

Automatic Translation

激光微解剖（LMD）是一种敏感且高度可重复的技术，可用于揭示介导胶质瘤异质性和入侵的途径。在这里，我们描述了一个优化的协议，使用激光LMD从胶质瘤组织中分离离散区域，然后进行转录分析。

Tags

Laser MicrodissectionRNA SequencingIntratumor HeterogeneityMesenchymal CellsTumor InvasionTumor MorphologyMolecular ProfilesTumor ProgressionTherapeutic TargetsBioluminescencePerfusionCryopreservationSpatial CharacterizationMolecular Characterization

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