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用于小鼠角膜和康康菲瓦实时成像的自定义多光子显微镜平台
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva
DOI:

06:53 min

May 17, 2020

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Chapters

  • 00:04Introduction
  • 00:40Multiphoton Microscopy Setup
  • 01:29Eye Holder Setup
  • 02:38Z-Serial Image Acquisition
  • 03:43Image Processing and 3D Reconstruction
  • 04:36Results: Representative Mouse Cornea and Conjunctiva Imaging
  • 06:10Conclusion

Summary

Automatic Translation

这里展示的是一个多光子显微平台,用于实时小鼠眼表面成像。荧光转基因小鼠能够可视化细胞核、细胞膜、神经纤维和眼表面的毛细血管。从胶原结构派生的非线性二谐波生成信号为频闪结构提供了无标签成像。

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