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En brugerdefineret multifoton mikroskopi platform for Live Imaging af mus hornhinde og konjunktiva
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1Department of Biomedical Engineering,National Taiwan University, 2Institute of Pharmacology, College of Medicine,National Taiwan University, 3Molecular Imaging Center,National Taiwan University, 4Department of Dermatology,National Taiwan University Hospital, and College of Medicine, 5Research Center for Developmental Biology and Regenerative Medicine,National Taiwan University, 6Department of Ophthalmology,Chang Gung Memorial Hospital, 7College of Medicine,Chang Gung University
Chapters
- 00:04Introduction
- 00:40Multiphoton Microscopy Setup
- 01:29Eye Holder Setup
- 02:38Z-Serial Image Acquisition
- 03:43Image Processing and 3D Reconstruction
- 04:36Results: Representative Mouse Cornea and Conjunctiva Imaging
- 06:10Conclusion
Præsenteret her er en multiphoton mikroskopisk platform for levende mus okulær overflade billeddannelse. Fluorescerende transgene mus muliggør visualisering af cellekerner, cellemembraner, nervefibre og kapillærer inden for den okulære overflade. Ikke-lineære anden harmoniske generation signaler stammer fra kollagen strukturer giver label-fri billeddannelse for stromale arkitekturer.
