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Chemistry
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用于从单一DNA分子大规模平行蛋白质合成的Femtoliter滴体阵列
JoVE Journal
Chemistry
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JoVE Journal
Chemistry
A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
Please note that all translations are automatically generated.
Click here for the English version.
用于从单一DNA分子大规模平行蛋白质合成的Femtoliter滴体阵列
DOI:
10.3791/60945-v
•
10:45 min
•
June 20, 2020
•
Yi Zhang
,
Kanako Kurosawa
,
Daisuke Nishiura
,
Mika Tei
,
Mikiko Tsudome
1
SUGAR Program, X-star
,
Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
,
2
Center for Mathematical Science and Advanced Technology
,
Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
,
3
Deep-Sea Nanoscience Research Group
,
Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
Chapters
00:04
Introduction
00:33
Cleaning and Silanizing the Glass
01:26
Spin-Coating the Glass Substrate
02:40
Photolithography, Etching, and Cleaning
03:47
Fabricating the Microchamber Array Device
05:04
Generating the Femtoliter Droplet Array (FemDA)
06:35
Image Data Analysis
08:45
Results: Cell-Free Protein Synthesis in FemDA
10:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议的总体目标是在 1 厘米
2
平面基板上准备超过 100 万个有序、均匀、稳定且生物相容的 femtoliter 液滴,可用于无细胞蛋白质合成。
Tags
Femtoliter Droplet Array
Single DNA Molecules
Parallel Protein Synthesis
Enzyme Activity Measurement
Microfabrication
Photolithography
CYTOP Polymer
Spin Coating
Reactive-ion Etching
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