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用于从单一DNA分子大规模平行蛋白质合成的Femtoliter滴体阵列
JoVE Journal
Chemistry
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JoVE Journal Chemistry
A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
DOI:

10:45 min

June 20, 2020

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Chapters

  • 00:04Introduction
  • 00:33Cleaning and Silanizing the Glass
  • 01:26Spin-Coating the Glass Substrate
  • 02:40Photolithography, Etching, and Cleaning
  • 03:47Fabricating the Microchamber Array Device
  • 05:04Generating the Femtoliter Droplet Array (FemDA)
  • 06:35Image Data Analysis
  • 08:45Results: Cell-Free Protein Synthesis in FemDA
  • 10:07Conclusion

Summary

Automatic Translation

该协议的总体目标是在 1 厘米2平面基板上准备超过 100 万个有序、均匀、稳定且生物相容的 femtoliter 液滴,可用于无细胞蛋白质合成。

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