/

Immunology and Infection

/

在小鼠中进行结肠镜引导捏活检并评估后续组织变化

JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Immunology and Infection

Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes

Please note that all translations are automatically generated. Click here for the English version.

在小鼠中进行结肠镜引导捏活检并评估后续组织变化

DOI:

10.3791/60949-v

•

08:52 min

•

February 05, 2021

•

David C. Montrose², Erin M. McNally, Erika Sue, Andrew J. Dannenberg

¹Department of Pathology, Renaissance School of Medicine,Stony Brook University, Stony Brook, NY, ²Stony Brook Cancer Center, Stony Brook, NY, ³Department of Medicine,Weill Cornell Medicine, New York, NY

Chapters

00:05Introduction
00:46Colonoscopy and Wound Induction
02:46Wound Bed Visualization and Measurement
04:37Wound Bed Collection
06:02Histological Analysis Preparation
07:45Results: Representative Wound Bed Visualization
08:21Conclusion

Summary

Automatic Translation

在这里，我们提供了一个详细的程序，诱导小鼠结肠镜引导捏活检和跟踪伤口关闭的实时程序。此外，还提供了组织准备方法，用于对伤口床进行组织学、免疫组织化学和分子分析。

Tags

Colonoscopic-guided Pinch Biopsy Mice Colonic Wound Healing Inflammatory Bowel Disease (IBD) Pathogenesis Endoscope Biopsy Forceps Anesthesia PBS Video Recording

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

右心室收缩压测量组合在小鼠的肺和免疫组织样品的收获

监测改变膜极性，膜完整性和细胞内离子浓度<em>肺炎链球菌</em>使用荧光染料

小鼠量度本地过敏反应

基于图像的流式细胞仪技术来评估变化粒细胞功能<em>在体外</em

方法来研究中性粒细胞脂质的改变和中性粒细胞胞外陷阱随后形成

人肺树突状细胞：空间分布与表型鉴定支气管活检采用免疫组化和流式细胞仪

线虫模型中气单胞菌感染的毒力及发病机制评价

小鼠动脉粥样硬化的定量

活细胞荧光显微镜研究细菌的亚细胞蛋白定位和细胞形态变化

葡萄糖-6-磷酸异构酶（G6PI）诱导RA小鼠中iNKT细胞的采用免疫治疗

Read Article