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En Neurite Outgrowth Assay og Neurotoxicity Vurdering med Human Neural Progenitor Cell-Derived Neuroner
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Developmental Biology
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JoVE Journal Developmental Biology
A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons

En Neurite Outgrowth Assay og Neurotoxicity Vurdering med Human Neural Progenitor Cell-Derived Neuroner

DOI:
10.3791/60955-v

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07:41 min

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August 06, 2020

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, , , ,
1Department of Molecular Genetics, Faculty of Biological Sciences,Tarbiat Modares University, 2Center for Therapeutic Innovation and Department of Psychiatry & Behavioral Sciences,University of Miami Miller School of Medicine, 3Department of Biochemistry and Molecular Biology,University of Miami Miller School of Medicine, 4Persian BayanGene Research and Training Center

Chapters

  • 00:04Introduction
  • 00:51Passaging and Freezing the hNPCs
  • 02:50Image Acquisition and Analysis
  • 04:25Neurotoxicity Assessment
  • 06:10Results: Neurite Outgrowth Assay and Neurotoxicity Assessment with Small Molecule Compounds
  • 07:13Conclusion

Summary

Automatic Translation

Automatic Translation

Den fremlagte protokol beskriver en metode til en neurite outgrowth assay og neurotoksicitet vurdering af små molekyle forbindelser.

Tags

Neurite Outgrowth AssayNeurotoxicity AssessmentHuman Neural Progenitor CellsNeuronsInduced Pluripotent Stem CellsEmbryonic Stem CellsNeurosphereCell DissociationCell CultureCryopreservationImage AnalysisFijiROI ManagerFreehand Line

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