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Developmental Biology
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神経前駆細胞由来ニューロンを用いた神経突起増殖アッセイと神経毒性評価
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons
Please note that all translations are automatically generated.
Click here for the English version.
神経前駆細胞由来ニューロンを用いた神経突起増殖アッセイと神経毒性評価
DOI:
10.3791/60955-v
•
07:41 min
•
August 06, 2020
•
Amir Bagheri
2
,
Seyedeh Fatemeh Razavipour
,
Claes Wahlestedt
,
Seyed Javad Mowla
,
Mohammad Ali Faghihi
4
1
Department of Molecular Genetics, Faculty of Biological Sciences
,
Tarbiat Modares University
,
2
Center for Therapeutic Innovation and Department of Psychiatry & Behavioral Sciences
,
University of Miami Miller School of Medicine
,
3
Department of Biochemistry and Molecular Biology
,
University of Miami Miller School of Medicine
,
4
Persian BayanGene Research and Training Center
Chapters
00:04
Introduction
00:51
Passaging and Freezing the hNPCs
02:50
Image Acquisition and Analysis
04:25
Neurotoxicity Assessment
06:10
Results: Neurite Outgrowth Assay and Neurotoxicity Assessment with Small Molecule Compounds
07:13
Conclusion
Summary
Automatic Translation
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Automatic Translation
提示されたプロトコルは、低分子化合物の神経突起成長アッセイおよび神経毒性評価のための方法を記述する。
Tags
Neurite Outgrowth Assay
Neurotoxicity Assessment
Human Neural Progenitor Cells
Neurons
Induced Pluripotent Stem Cells
Embryonic Stem Cells
Neurosphere
Cell Dissociation
Cell Culture
Cryopreservation
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