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Biochemistry

Karakterisering af Amyloid strukturer i Aging C. Elegans Brug Fluorescens Lifetime Imaging

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Biochemistry

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JoVE Journal Biochemistry

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

Karakterisering af Amyloid strukturer i Aging C. Elegans Brug Fluorescens Lifetime Imaging

DOI:

10.3791/61004-v

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09:31 min

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March 27, 2020

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Maria Lucia Pigazzini*^1,2, Christian Gallrein*¹, Manuel Iburg*¹, Gabriele Kaminski Schierle, Janine Kirstein⁴

¹Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund Berlin, ²NeuroCure Cluster of Excellence,Charité – Universitätsmedizin Berlin, ³Molecular Neuroscience Group, Department of Chemical Engineering and Biotechnology,University of Cambridge, ⁴Cell Biology,University of Bremen

Chapters

00:05Introduction
01:25Synchronization of C. elegans and Mounting Nematodes onto Microscopy Slides
02:58Acquisition of FLIM (Fluorescence Lifetime Imaging Microscopy) Data
04:32Analysis of FLIM Data Using FLIMfit Software
07:15Results: Fluorescence Lifetimes
08:15Conclusion

Summary

Automatic Translation

Fluorescens levetid imaging overvåger, kvantificerer og skelner aggregering tendenser af proteiner i levende, aldring, og understregede C. elegans sygdom modeller.

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