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Immunology and Infection
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多能干细胞人类巨噬细胞的产生与表征
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Production and Characterization of Human Macrophages from Pluripotent Stem Cells
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多能干细胞人类巨噬细胞的产生与表征

DOI:
10.3791/61038-v

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08:05 min

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April 16, 2020

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, , , , , , ,
1Centre for Regenerative Medicine, Scottish Centre for Regenerative Medicine,University of Edinburgh, 2Centre for Reproductive Health, The Queen’s Medical Research Institute,University of Edinburgh

Chapters

  • 00:04Introduction
  • 00:51Passaging Human iPSCs
  • 01:46Human iPSC Differentiation to Macrophages
  • 04:33High-Throughput Phagocytosis Assay
  • 05:07Results: Phenotype and Function of iPSC-Derived Macrophages
  • 07:08Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了人类诱导多能干细胞的巨噬细胞的强健生成，以及其后续表征的方法。细胞表面标记表达、基因表达和功能测定用于评估这些iPSC衍生巨噬细胞的表型和功能。

Tags

MacrophagePluripotent Stem CellsCell TherapyGenetic ManipulationCell CultureCell DifferentiationEmbryoid BodiesCell PassagingCell HarvestingMedia Replenishment

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