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Dobbelt i Utero elektroporation til target temporally og rumligt adskilte cellepopulationer
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Chapters
- 00:04Introduction
- 01:09First In Utero Electroporation Surgery
- 03:20Second In Utero Electroporation
- 04:18Tissue Harvesting and Sectioning: Strategy A
- 06:09Tissue Harvesting and Sectioning: Strategy B
- 07:24Confocal Fluorescence Imaging and Analysis
- 08:34Results: Representative Double Electroporation-Facilitated Cell Interaction Visualization Strategies
- 09:55Conclusion
Dobbelt i livmoderen elektroporation tillader målretning cellepopulationer, der er rumligt og tidsmæssigt adskilt. Denne teknik er nyttig til at visualisere interaktioner mellem disse cellepopulationer ved hjælp af fluorescerende proteiner under normale forhold, men også efter funktionelle eksperimenter for at forstyrre gener af interesse.
Tags
Double In Utero ElectroporationCorticogenesisNeuron CellsNeuronal DisordersAutismSchizophreniaLateral VentriclesFast Green DyePlasmid DNAAnesthetized Pregnant MouseRazor70% EthanolIodine WipesRing ForcepsMouth Controlled Aspirator TubeLateral VentricleSquare-wave ElectroporaterCortical HemLateral Cortex
