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Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
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Genetics
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JoVE Journal Genetics
Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

DOI:
10.3791/61060-v

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07:30 min

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June 08, 2020

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, , , , ,
1NCI CCR Sequencing Facility,Frederick National Laboratory for Cancer Research, 2Advanced Biomedical and Computational Sciences,Frederick National Laboratory for Cancer Research

Chapters

  • 00:04Introduction
  • 01:21RNA Quantity and Quality Assessment
  • 02:03Sequencing
  • 04:01Data Analysis and Quality Assessment
  • 04:33Results: Representative Degraded Formalin-Fixed Paraffin Embedded (FFPE)- RNA Sample Sequencing and Analysis
  • 06:28Conclusion

Summary

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This method describes the steps to improve the quality and quantity of sequence data that can be obtained from formalin-fixed paraffin-embedded (FFPE) RNA samples. We describe the methodology to more accurately assess the quality of FFPE-RNA samples, prepare sequencing libraries, and analyze the data from FFPE-RNA samples.

Tags

Keywords: FFPE-RNARNA QualityRNA DegradationSample QCLibrary PreparationSequencing ProcessRNA FragmentsSequencing ReagentsFlow CellSample LibraryPhiX Control

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