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Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

DOI:
10.3791/61086-v

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08:37 min

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October 09, 2020

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1Development, Disease Models & Therapeutics Graduate Program, Department of Molecular and Cellular Biology, Department of Neuroscience, Center for Cell and Gene Therapy, Stem Cells and Regenerative Medicine Center,Baylor College of Medicine

Chapters

  • 00:04Introduction
  • 01:00Embryo Dissection
  • 01:51Embryo Dissociation and Cell Sorting
  • 03:11Size Selection
  • 06:02Results: Small RNA Libraries
  • 07:53Conclusion

Summary

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We describe a technique for profiling microRNAs in early mouse embryos. This protocol overcomes the challenge of low cell input and small RNA enrichment. This assay can be used to analyze changes in miRNA expression over time in different cell lineages of the early mouse embryo.

Tags

Small RNA SequencingMicroRNAsEmbryonic DevelopmentLow Input SamplesEmbryo DissectionEmbryo StagingCell SuspensionCell SortingRNA Extraction

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