JoVE Journal
Chemistry
Chemistry
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
The goal of the thiobarbituric acid reactive substances assay is to assess oxidative stress in biological samples by measuring the production of lipid peroxidation products, primarily malondialdehyde, using visible wavelength spectrophotometry at 532 nm. The method described here can be applied to human serum, cell lysates, and low density lipoproteins.