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Isolering og karakterisering af exosomer fra skeletmuskelfibroblaster
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JoVE Journal Biology
Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts

Isolering og karakterisering af exosomer fra skeletmuskelfibroblaster

DOI:
10.3791/61127-v

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06:27 min

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May 16, 2020

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, , ,
1Department of Genetics,St. Jude Children’s Research Hospital

Chapters

  • 00:05Introduction
  • 00:45Primary Fibroblast Isolation and Culture
  • 03:04Purification of Exosomes Using Differential and Ultra-Centrifugation
  • 04:58Results: Characterization of Purified Exosomes
  • 05:47Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol illustrerer 1) isolering og dyrkning af primære fibroblaster fra den voksne muses gastrocnemiusmuskel samt 2) oprensning og karakterisering af exosomer ved hjælp af en differentiel ultracentrifugeringsmetode kombineret med saccharosedensitetsgradienter efterfulgt af western blot-analyser.

Tags

ExosomesSkeletal Muscle FibroblastsDifferential CentrifugationIsolationCharacterizationBiomarkersCell CultureLow Oxygen Conditions

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