Journal
/
Immunology and Infection
/
Undersøgelse virkninger af cigaretrøg på Pseudomonas infektion i lung epitelceller
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells

Undersøgelse virkninger af cigaretrøg på Pseudomonas infektion i lung epitelceller

DOI:
10.3791/61163-v

•

09:15 min

•

May 11, 2020

•

, , ,
1Acute Lung Injury Center of Excellence, Pulmonary, Allergy, Critical Care Medicine, Department of Medicine,University of Pittsburgh School of Medicine

Chapters

  • 00:04Introduction
  • 00:42Cigarette Smoking Extract (CSE) Preparation
  • 01:39Pseudomonas Culture
  • 02:16Cell Culture and CSE Treatment
  • 03:33Bacterial Infection and Gentamycin Treatment
  • 04:24Determination of Bacterial Concentration Using the Drop Plate Method
  • 04:55RT-qPCR Detection of Bacterial 16S rRNA
  • 07:00Results: CSE-Treatment Increases Bacterial Load
  • 08:23Conclusion

Summary

Automatic Translation

Automatic Translation

Beskrevet her er en protokol til at undersøge, hvordan cigaretrøg ekstrakt påvirker bakteriel kolonisering i lunge epitelceller.

Tags

Cigarette SmokePseudomonasLung Epithelial CellsBacterial LoadCell CultureSmoke ExtractionBacterial InfectionCell CountingCell SuspensionCell Preparation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/61163?article_type=v

Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells
check_url/61163?article_type=v

Anvendelse af en mus Ligated Peyer er Patch Intestinal Loop Assay at evaluere Bakteriel Optagelse ved M celler
check_url/61163?article_type=v

Brug af kunstig Sputum Medium til Test antibiotikum Effekt mod<em> Pseudomonas aeruginosa</em> I forhold mere relevante for cystisk fibrose Lung
check_url/61163?article_type=v

Flowcytometrisk Isolering af primære murine type II alveolære epitelceller for Funktionelle og molekylære studier
check_url/61163?article_type=v

Langtids kronisk<em> Pseudomonas aeruginosa</em> Luftvejsinfektion i mus
check_url/61163?article_type=v

Pseudomonas aeruginosa Induced Lung Injury Model
check_url/61163?article_type=v

At analysere virkningen af ​​stromacellers om ansættelse af Leukocytter fra Flow
check_url/61163?article_type=v

Flow cytometri af partikelbundet Bet v 1 Allergen i PM10
check_url/61163?article_type=v

Visualisere virkningerne af sputum på Biofilm Development Ved hjælp af en Chambered dækglas Model
check_url/61163?article_type=v

Vurdering af den cytotoksiske og immunmodulerende virkninger af stoffer i menneskelige præcision-cut lunge skiver

Read Article