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Immunology and Infection
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分离中枢神经系统组织和相关脑膜,用于免疫细胞的下游分析
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Immunology and Infection
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Immunology and Infection
Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells
Please note that all translations are automatically generated.
Click here for the English version.
分离中枢神经系统组织和相关脑膜,用于免疫细胞的下游分析
DOI:
10.3791/61166-v
•
09:35 min
•
May 19, 2020
•
Krista D. DiSano
,
Michael R. Linzey
2
,
Nora C. Welsh
2
,
Joshua S. Meier
,
Andrew R. Pachner
,
Francesca Gilli
1
Department of Neurology, Geisel School of Medicine
,
Dartmouth-Hitchcock Medical Center
,
2
Program in Experimental and Molecular Medicine
,
Dartmouth College
Chapters
00:05
Introduction
00:53
Processing Brain and Spinal Cord Samples
02:01
Preparation of the Meninges and CNS Tissues for Flow Cytometry Staining
04:33
Preparation of Single‐Cell Suspensions of Brain and Spinal Cord Tissue
06:57
Results: Immune Cells in CNS Tissues of TMEV‐IDD Mice
08:50
Conclusion
Summary
Automatic Translation
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本文提出了两种优化的方案,用于检查中枢神经系统内的常驻和外周来源的免疫细胞,包括大脑、脊髓和脑膜。这些协议中的每一个都有助于确定在稳态和炎症条件下占据这些隔室的细胞的功能和组成。
Tags
Meninges
Immune Cells
Single Cell Analysis
Histological Methods
Microglia
Astrocytes
Pericytes
Endothelial Cells
Stromal Cells
Neurons
Brain
Spinal Cord
Decalcification
Skull Cap
Vertebrae Column
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