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Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation
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1Department of Microbiology, Immunology and Molecular Genetics, Long School of Medicine,University of Texas Health San Antonio, 2Department of Clinical Laboratory Sciences, College of Applied Medical Sciences,Jouf University, 3Department of Otolaryngology-Head and Neck Surgery,Xiangya Hospital, Central South University
Chapters
- 00:04Introduction
- 01:02Dot Blot to Show the Presence of dsRNA Structures in HDM Total RNA
- 03:54FACS Analysis to Determine the Effects of HDM RNA on the Infiltration of Eosinophils in BAL and Lung
- 06:19Results: dsRNA Structures in HDM RNAs
- 07:53Conclusion
Environmental allergens such as house dust mites (HDM) often contain microbial substances that activate innate immune responses to regulate allergic inflammation. The protocol presented here demonstrates the identification of dsRNA species in HDM allergens and characterization of their immunogenic activities in modulating eosinophilic lung inflammation.
