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Induction of Diffuse Axonal Brain Injury in Rats Based on Rotational Acceleration
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JoVE Journal Neuroscience
Induction of Diffuse Axonal Brain Injury in Rats Based on Rotational Acceleration

Induction of Diffuse Axonal Brain Injury in Rats Based on Rotational Acceleration

DOI:
10.3791/61198-v

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06:14 min

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May 09, 2020

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, , , , , , , , ,
1Division of Anesthesia and Critical Care, Soroka University Medical Center and the Faculty of Health Sciences,Ben-Gurion University of the Negev, 2Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences,Ben-Gurion University of the Negev, 3Department of Anesthesiology,Yale University School of Medicine, 4Recanati School for Community Health Professions, Faculty of Health Sciences,Ben-Gurion University of the Negev, 5Department of microbiology and immunology, Faculty of Health Sciences,Ben-Gurion University of the Negev

Chapters

  • 00:04Introduction
  • 00:45Preparing Rats for the Experimental Procedure
  • 01:11Procedure of Induction Diffuse Axonal Brain Injury
  • 01:54Measurement of Rotational Kinematics, Biomechanical Parameters
  • 02:32Evaluation of Neurological Severity Score after 48 hours
  • 03:08Brain Collection for Histological Examination after 48 hours
  • 04:02Immunohistochemical Staining
  • 04:16Results
  • 05:22Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol validates a reliable, easy-to-perform and reproducible rodent model of brain diffuse axonal injury (DAI) that induces widespread white matter damage without skull fractures or contusions.

Tags

Diffuse Axonal Brain InjuryTraumatic Brain InjuryRotational AccelerationSprague-Dawley RatsNeurological Severity ScoreBeta-amyloid Precursor ProteinImmunochemical StainingBrain Injury Model

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