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Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications
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Biology
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JoVE Journal Biology
Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

DOI:
10.3791/61199-v

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06:34 min

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March 08, 2024

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, , , , ,
1Biodiscovery Institute, Translational Medical Sciences, School of Medicine,University of Nottingham

Chapters

  • 00:00Introduction
  • 00:32Intestinal Organoid Passaging
  • 01:59Passaging and Intestinal Organoid Stimulation
  • 04:12Results: hiPSC to Hindgut (HG) Endoderm Differentiation Characterization
  • 06:10Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol allows for the differentiation of human pluripotent cells into intestinal organoids. The protocol mimics normal human development by differentiating cells into a population of definitive endoderm, hindgut endoderm and then intestinal epithelium. This makes the protocol suitable for studying both intestinal development as well as disease modelling applications.

Tags

HiPSCIntestinal OrganoidsDevelopmental ModelingDisease ModelingDefinitive EndodermHindgut Epithelium3D CultureExtracellular MatrixGrowth FactorsCentrifugationDissociationTranslatability

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