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Developmental Biology
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研究伤口引起的
多倍体化的果蝇模型
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
A
Drosophila
Model to Study Wound-induced Polyploidization
Please note that all translations are automatically generated.
Click here for the English version.
研究伤口引起的
多倍体化的果蝇模型
DOI:
10.3791/61252-v
•
07:27 min
•
June 09, 2020
•
Erin C. Bailey*
1
,
Ari S. Dehn*
1
,
Kayla J. Gjelsvik
,
Rose Besen-McNally
,
Vicki P. Losick
1
Biology Department
,
Boston College
,
2
Graduate School of Biomedical Sciences and Engineering and Kathryn W. Davis Center for Regenerative Biology and Medicine, MDI Biological Laboratory
,
University of Maine
Chapters
00:04
Introduction
00:32
Adult Fruit Fly Staging and Wounding
01:49
Fly Abdomen Dissection
04:00
Stained Tissue Mounting
04:57
Results: Representative Re-Epithelization and Endoreplication Detection
06:47
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
伤口诱导的多倍体化是一种保守的组织修复策略,其中细胞在大小上生长,而不是分裂,以弥补细胞损失。下面是一个详细的协议,如何使用果蝇作为模型来测量皮骨及其遗传调控在上皮伤口修复。
Tags
Drosophila
Wound-induced Polyploidization
Epithelial Cells
Ploidy
Abdominal Wounding
Grace’s Solution
Dissection
Microscopy
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