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Forward Genetiske Screen Brug Transgenic Calcium Reporter Aequorin at identificere nye mål i Calcium Signalering
JoVE Journal
Biology
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JoVE Journal Biology
Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling
DOI:

08:46 min

August 01, 2020

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Chapters

  • 00:00Introduction
  • 00:52EMS Mutagenesis (1-3 months)
  • 03:10High Throughput Screening (8 months)
  • 05:21Data Analysis and Results (1-3 months)
  • 06:46Conclusion and Final Thoughts

Summary

Automatic Translation

En fremadrettet genetisk skærm baseret på Ca2 + elevation som en udlæst fører til identifikation af genetiske komponenter involveret i calcium afhængige signalering veje i planter.

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