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Immunology and Infection
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Phage Therapy Application to Counteract Pseudomonas aeruginosa Infection in Cystic Fibrosis Zebrafish Embryos
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Immunology and Infection
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JoVE Journal Immunology and Infection
Phage Therapy Application to Counteract Pseudomonas aeruginosa Infection in Cystic Fibrosis Zebrafish Embryos

Phage Therapy Application to Counteract Pseudomonas aeruginosa Infection in Cystic Fibrosis Zebrafish Embryos

DOI:
10.3791/61275-v

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11:20 min

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May 12, 2020

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, , , ,
1Dipartimento di Scienze Cliniche e Comunità,Università degli Studi di Milano, 2Dipartimento di Biotecnologie Mediche e Medicina Traslazionale,Università degli Studi di Milano, LITA, 3Dipartimento di Bioscienze,Università degli Studi di Milano

Chapters

  • 00:04Introduction
  • 00:48Phage Stock and Phage Cocktail Preparation
  • 03:45Zebrafish Embryo Cystic Fibrosis Transmembrane Conductance Regulator (cftr) Morpholino Microinjection
  • 04:51Zebrafish Embryo P. aeruginosa (PAO1) Bacteria and Phage Cocktail Microinjection
  • 06:12Time-Lapse Imaging Preparation
  • 07:06Bacterial Burden and PAO1 Infection Embryonic Lethality Evaluation
  • 08:48Results: Representative Phage Therapy Effects on Pseudomonas aeruginosa Infection in Cystic Fibrosis (CF) Zebrafish Embryos
  • 10:36Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a protocol for Pseudomonas aeruginosa infection and phage therapy application in cystic fibrosis (CF) zebrafish embryos.

Tags

Phage TherapyPseudomonas AeruginosaCystic FibrosisZebrafish EmbryosPhage CocktailIn Vivo AssessmentBacterial InfectionPhage Stock PreparationMultiplicity Of InfectionDNaseRNaseCentrifugationCesium Chloride Density GradientUltracentrifugation

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