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动脉瘤高脂血症小鼠模型中油红O染色全主动脉病变的成像和分析
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Medicine
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JoVE Journal
Medicine
Imaging and Analysis of Oil Red O-Stained Whole Aorta Lesions in an Aneurysm Hyperlipidemia Mouse Model
Please note that all translations are automatically generated.
Click here for the English version.
动脉瘤高脂血症小鼠模型中油红O染色全主动脉病变的成像和分析
DOI:
10.3791/61277-v
•
07:57 min
•
May 02, 2022
•
Pei-Yu Chen
,
Lingfeng Qin
,
Michael Simons
3
1
Yale Cardiovascular Research Center, Department of Internal Medicine
,
Yale University School of Medicine
,
2
Department of Surgery
,
Yale University School of Medicine
,
3
Department of Cell Biology
,
Yale University School of Medicine
Chapters
00:05
Introduction
00:38
Heart Perfusion
01:39
Aorta and Aortic Branch Isolation
02:36
Heart and Aorta Fixation
03:29
Unopened Whole Aorta Oil Red O Staining and Imaging
04:43
En Face Aorta Mounting
05:39
Results: Representative Atherosclerotic Lesion Visualization and Quantification
06:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议提供了
分析小鼠动脉粥样硬化负担的分步程序。研究人员可以使用该协议来比较不同动物中动脉粥样硬化病变的丰度,位置和大小。
Tags
Aorta
Atherosclerosis
Aneurysm
Hyperlipidemia
Mouse Model
Oil Red O Staining
Perfusion
Dissection
Fixation
Quantification
Vascular Lesions
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