A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:00Introduction
- 02:33Isolation of Mice TDSCs
- 03:30Culture and Expansion of Mice TDSCs
- 05:12Preparation of Stimulation Culture Medium for Cell Sheet Formation
- 05:44Cell Sheet Formation by Culturing in Stimulation Medium
- 06:21Preparation of 3D Tendon Stem Cell Construct
- 07:50Assembly of the Uniaxial-Stretching Mechanical Stimulation Complex in Unique designed Bioreactor
- 09:25Evaluation of the Mechanical Stimulated in vitro Tendon-like Tissue
- 10:10Representative Results
- 11:07Discussion
