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Beeldvorming Intranucleaire Actin Staven in Live Heat Stressed Drosophila Embryo's

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Developmental Biology

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JoVE Journal Developmental Biology

Imaging Intranuclear Actin Rods in Live Heat Stressed Drosophila Embryos

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Beeldvorming Intranucleaire Actin Staven in Live Heat Stressed Drosophila Embryo's

DOI:

10.3791/61297-v

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07:57 min

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May 15, 2020

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Natalie Biel², Lauren Figard, Anna Marie Sokac^2,3

¹Integrative Molecular and Biomedical Sciences,Baylor College of Medicine, ²Department of Cell and Molecular Biology, School of Molecular and Cellular Biology,University of Illinois at Urbana-Champaign, ³Verna and Marrs McLean Department of Biochemistry and Molecular Biology,Baylor College of Medicine

Chapters

00:04Introduction
00:51Embryo Collection and Mounting
03:43Injection and Heat Stress to Promote Rod Formation
04:45Imaging of Actin Rods with Confocal Microscopy
06:32Results: Actin Rods in Heat-stressed Embryos
07:17Conclusion

Summary

Automatic Translation

Het doel van dit protocol is om Rhodamine-geconjugeerde bolvormige actine te injecteren in Drosophila embryo's en beeld intranucleaire actine staaf assemblage na hitte stress.

Tags

Intranuclear Actin Rods Live Heat Stressed Drosophila Embryos Microinjection Actin Stress Response Actin Rod Assembly Mutant Screen Stress Conditions Embryo Handling Embryo Positioning Embryo Mounting G-actin Red Apple Juice Agar Dechorionation Embryo Transfer Embryo Alignment Embryo Glue Coverslip Mounting

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