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Automation of Bio-Atomic Force Microscope Measurements on Hundreds of C. albicans Cells
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JoVE Journal Bioengineering
Automation of Bio-Atomic Force Microscope Measurements on Hundreds of C. albicans Cells

Automation of Bio-Atomic Force Microscope Measurements on Hundreds of C. albicans Cells

DOI:
10.3791/61315-v

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09:27 min

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April 02, 2021

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, , , ,
1ITAV-CNRS,Université de Toulouse, CNRS, Toulouse, France, 2LAAS-CNRS,Université de Toulouse, CNRS, Toulouse, France, 3ENCB, Instituto Politécnico Nacional (IPN), Mexico City, Mexico, 4TBI, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France, 5CIC, Instituto Politécnico Nacional (IPN), Mexico City, Mexico

Chapters

  • 00:04Introduction
  • 01:03Polydimethylsiloxane (PDMS) Stamp Preparation
  • 02:06Cell Immobilization
  • 03:21Atomic Force Microscopy (AFM) Setup
  • 03:58AFM Imaging
  • 06:26Data Analysis
  • 07:45Results: Representative Median Stiffness Analyses
  • 08:50Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol aims to automate AFM measurements on hundreds of microbial cells. First, microbes are immobilized into PDMS stamp microstructures and then force spectroscopy measurements are performed automatically on hundreds of immobilized cells.

Tags

Keywords: Atomic Force Microscope AutomationBiomedical ApplicationsBiomechanical PropertiesCell PopulationsC. Albicans CellsPDMS StampCell SuspensionMicrostructuresCell TrappingAFM Measurements

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