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Double Direct Injection of Blood into the Cisterna Magna as a Model of Subarachnoid Hemorrhage
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JoVE Journal Neuroscience
Double Direct Injection of Blood into the Cisterna Magna as a Model of Subarachnoid Hemorrhage

Double Direct Injection of Blood into the Cisterna Magna as a Model of Subarachnoid Hemorrhage

DOI:
10.3791/61322-v

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10:34 min

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August 30, 2020

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, , , ,
1University of Rouen Normandy, INSERM U1239, DC2N, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France, 2Department of Anesthesiology and Critical Care,Rouen University Hospital, Rouen, France

Chapters

  • 00:00Introduction
  • 02:07Procedure: Setup of SAH Surgery
  • 02:47Procedure: Collection of Blood from a Homologous Mouse Donor
  • 03:25Procedure: Animal Preparation
  • 04:21Procedure: SAH Induction
  • 06:18Post-surgery Procedure
  • 06:42Second Day of Surgery
  • 07:38Representative Results
  • 09:51Conclusion

Summary

Automatic Translation

Automatic Translation

We described in this protocol a standardized subarachnoid hemorrhage (SAH) mouse model by a double injection of autologous whole-blood into the cisterna magna. The high degree of standardization of the double-injection procedure represents a middle-to-acute model of SAH with relative safety regarding mortality.

Tags

Keywords: Subarachnoid HemorrhageDouble Direct InjectionCisterna MagnaAneurysmCerebral IschemiaVasospasmAnimal ModelAutologous Arterial BloodNoninvasive Surgical ProcedureIntracranial Pressure

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