Journal
/
/
使用 德罗索菲拉· 拉瓦在恒温遗传神经元屏幕上摄入的宏量营养素的量化
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae
DOI:

07:24 min

June 11, 2020

, ,

Chapters

  • 00:04Introduction
  • 00:32Preparation of Third-instar Larvae (L3)
  • 02:24Thermogenetic Activation and No-choice Feeding Assay
  • 03:35Food Dye Extraction and Colorimetric Quantification of Food Consumption
  • 05:37Results: Amount of Food Eaten per Larva under Neuronal Thermogenetic-activation Conditions in Three Macronutrient Balancing Diets
  • 06:49Conclusion

Summary

Automatic Translation

这里描述的是一个协议,使颜色定量在定义的时间间隔内食用的食物量,由 德罗索菲拉梅拉诺加斯特 幼虫暴露在不同宏量营养素品质的饮食。这些检测是在神经元恒温屏幕的背景下进行的。

Related Videos

Read Article