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Kvantificering af makronæringsstoffer Indtag i en termogentisk neuronal skærm ved hjælp af Drosophila Larver
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae
DOI:

07:24 min

June 11, 2020

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Chapters

  • 00:04Introduction
  • 00:32Preparation of Third-instar Larvae (L3)
  • 02:24Thermogenetic Activation and No-choice Feeding Assay
  • 03:35Food Dye Extraction and Colorimetric Quantification of Food Consumption
  • 05:37Results: Amount of Food Eaten per Larva under Neuronal Thermogenetic-activation Conditions in Three Macronutrient Balancing Diets
  • 06:49Conclusion

Summary

Automatic Translation

Beskrevet her er en protokol, der muliggør den kolorimetriske kvantificering af mængden af mad, der spises inden for et defineret tidsinterval af Drosophila melanogaster larver udsat for kost af forskellig makronæringskvalitet. Disse assays udføres i forbindelse med en neuronal termogentisk skærm.

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