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从成人和老化小鼠的河马切片中尽量减少低氧
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Neuroscience
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JoVE Journal Neuroscience
Minimizing Hypoxia in Hippocampal Slices from Adult and Aging Mice
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从成人和老化小鼠的河马切片中尽量减少低氧

DOI:
10.3791/61377-v

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08:58 min

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July 02, 2020

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1Department of Biology, Neurobiology, and Bio-X,Stanford University

Chapters

  • 00:04Introduction
  • 01:13Setup
  • 02:33Transcardial Perfusion and Brain Extraction
  • 04:57Slicing and Recovery
  • 06:24Results: Patch-clamp and Synaptic Plasticity Measurements in Hippocampal Slices from Mice at P120 180
  • 07:57Conclusion

Summary

Automatic Translation

Automatic Translation

这是一个急性切片制备从成人和老化的小鼠海马，利用转心灌注和切片切割与冰冷的NMDG-aCSF，以减少对组织的低氧损伤。生成的切片在几个小时内保持健康，并适用于长期的贴片夹和现场记录。

Tags

Hippocampal SlicesAdultAgingHypoxiaHypothermiaNMDG-aCSFTranscardial PerfusionVibrating MicrotomeField-recordingsPatch-clampNeurodegenerative Diseases

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