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Neuroscience
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从成人和老化小鼠的河马切片中尽量减少低氧
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Neuroscience
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JoVE Journal
Neuroscience
Minimizing Hypoxia in Hippocampal Slices from Adult and Aging Mice
Please note that all translations are automatically generated.
Click here for the English version.
从成人和老化小鼠的河马切片中尽量减少低氧
DOI:
10.3791/61377-v
•
08:58 min
•
July 02, 2020
•
Maja Djurisic
1
Department of Biology, Neurobiology, and Bio-X
,
Stanford University
Chapters
00:04
Introduction
01:13
Setup
02:33
Transcardial Perfusion and Brain Extraction
04:57
Slicing and Recovery
06:24
Results: Patch-clamp and Synaptic Plasticity Measurements in Hippocampal Slices from Mice at P120 180
07:57
Conclusion
Summary
Automatic Translation
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Automatic Translation
这是一个急性切片制备从成人和老化的小鼠海马,利用转心灌注和切片切割与冰冷的NMDG-aCSF,以减少对组织的低氧损伤。生成的切片在几个小时内保持健康,并适用于长期的贴片夹和现场记录。
Tags
Hippocampal Slices
Adult
Aging
Hypoxia
Hypothermia
NMDG-aCSF
Transcardial Perfusion
Vibrating Microtome
Field-recordings
Patch-clamp
Neurodegenerative Diseases
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