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监测三D培养中的癌细胞入侵和T细胞毒性
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Cancer Research
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JoVE Journal Cancer Research
Monitoring Cancer Cell Invasion and T-Cell Cytotoxicity in 3D Culture
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监测三D培养中的癌细胞入侵和T细胞毒性

DOI:
10.3791/61392-v

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07:44 min

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June 23, 2020

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, , , , , , ,
1Lombardi Comprehensive Cancer Center, Department of Oncology,Georgetown University

Chapters

  • 00:04Introduction
  • 00:37Spheroid Generation
  • 01:43T Cell Co-Culture
  • 02:393D Co-Culture Embedding
  • 03:41Immunofluorescence StainingImmunofluorescence Staining
  • 05:43Results: Representative Cancer Cell Invasion Monitoring
  • 07:18Conclusion

Summary

Automatic Translation

Automatic Translation

该方法同时评价了三D球类测定和T细胞毒性中的癌细胞入侵。球体在无脚手架的阿加罗斯多微井铸件中生成。I型胶原蛋白基质中的共同培养和嵌入在同一设备中进行，允许监测癌细胞入侵和T细胞中角细胞毒性。

Tags

3D CultureCancer Cell InvasionT-cell CytotoxicitySpheroid Co-cultureAgarose MoldTumor Cell SuspensionT-cell Co-cultureType I Collagen3D Co-cultureMechanistic InsightsImmune Cell-mediated Tumor Cell CytotoxicityInvasive Behavior

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