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Superoppløsningsavbildning for å studere samtidig lokalisering av proteiner og synaptiske markører i primære nevroner
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Neuroscience
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JoVE Journal Neuroscience
Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Superoppløsningsavbildning for å studere samtidig lokalisering av proteiner og synaptiske markører i primære nevroner

DOI:
10.3791/61434-v

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14:02 min

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October 31, 2020

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, , , , , , , ,
1Department of Molecular Biochemistry and Pharmacology,Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 2Department of Neuroscience,Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 3Dulbecco Telethon Institute,Istituto di Ricerche Farmacologiche Mario Negri IRCCS

Chapters

  • 00:00Introduction
  • 01:04Primary Cultures
  • 01:48Immunofluorescence Staining
  • 03:32SIM Microscopy
  • 04:01Microscope Calibration
  • 07:11Acquisition
  • 09:24Post-production: Image Reconstruction
  • 11:24Colocalization with Profile Analysis
  • 12:05Quantification of Pearson’s and Mander’s
  • 12:27Results
  • 13:16Conclusions

Summary

Automatic Translation

Automatic Translation

Denne protokollen viser hvordan du bruker superoppløsningsmikroskopi for å studere proteinkon-lokalisering i primære nevronale kulturer.

Tags

Super-resolution ImagingCo-localizationPrimary NeuronsSynaptic MarkersStructured Illumination Microscopy (SIM)Neurodegenerative DisordersHippocampal NeuronsFixationImmunostainingMounting SolutionCoverglass

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