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En enkelt celle dissociation tilgang til molekylær analyse af urinblæren i musen efter rygmarvsskade
 
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En enkelt celle dissociation tilgang til molekylær analyse af urinblæren i musen efter rygmarvsskade

Article DOI: 10.3791/61455-v • 09:50 min • June 17th, 2020
June 17th, 2020 •
*1,2, *1,2, 1, 3,4,5, 1,6, 1,2, 2,7, 2,7, 1,2
1Department of Urology, Boston Children's Hospital, 2Department of Surgery, Harvard Medical School, 3Division of Hematology/Oncology, Harvard Medical School Boston, 4Dana-Farber Cancer Institute, 5Broad Institute, 6Biological Biomedical Sciences Program, Division of Medical Sciences, Harvard Medical School, 7Vascular Biology Program, Boston Children's Hospital
* These authors contributed equally

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Målet med denne protokol er at anvende en optimeret væv dissociation protokol til en mus model af rygmarvsskader og validere tilgangen til enkelt celle analyse ved flow cytometry.

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Biologi Problem 160 Rygmarv skade enkelt celle dissociation flow cytometry murine
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