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Journal / Biochemistry / Ekstraktion og kvantificering af opløselige,…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Ekstraktion og kvantificering af opløselige, radioaktivt mærkede inositolpolyfosfater fra forskellige plantearter ved hjælp af SAX-HPLC
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Ekstraktion og kvantificering af opløselige, radioaktivt mærkede inositolpolyfosfater fra forskellige plantearter ved hjælp af SAX-HPLC

Article DOI: 10.3791/61495-v • 09:01 min • June 26th, 2020
June 26th, 2020 •
1, 1, 1, 1
1Department of Plant Nutrition, Institute of Crop Science and Resource Conservation, University of Bonn

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Her beskriver vi stærk anionudveksling højtydende væskekromatografi af [3H]- myo-inositol-mærkede planter, som er en meget følsom metode til at detektere og kvantificere inositolpolyphosphater i planter.myo

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Denne måned i JoVE Stærk anion udveksling kromatografi (SAX)-HPLC radiomærkning Arabidopsis thaliana inositol polyfosfater (InsPs) inositol pyrophosphater (PP-InsPs) signalering molekyler Lotus japonicus plante forsvar jasmonat opfattelse fosfat sult svar energi homøostase
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