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1Institute of Integrated Clinical Practice, Faculty of Medicine,University of Coimbra, Coimbra, Portugal, 2Coimbra Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO),University of Coimbra, Coimbra, Portugal, 3Center for Innovative Biomedicine and Biotechnology (CIBB),University of Coimbra, Coimbra, Portugal, 4Clinical Academic Center of Coimbra, CACC, Portugal, 5Institute of Biophysics, Faculty of Medicine,University of Coimbra, Coimbra, Portugal, 6Laboratory of Oncobiology and Hematology, Faculty of Medicine,University of Coimbra, Coimbra, Portugal, 7Institute of Endodontics, Faculty of Medicine,University of Coimbra, Coimbra, Portugal, 8Institute of Experimental Pathology, Faculty of Medicine,University of Coimbra, Coimbra, Portugal
Chapters
- 00:05Introduction
- 01:12Pellet Preparation and Soluble Biomaterial Extracts Acquisition
- 02:11Morphology Evaluation
- 03:28Cell Function Assessment
- 05:54Mineralized Formation Assessment
- 06:50Results: Cell Viability, Morphology, and Protein Expression in Response to Biomaterial Exposure
- 08:30Conclusion
Deze methodologie is gericht op het evalueren van biomateriaal cytotoxiciteit door de bereiding van oplosbare extracten, met behulp van levensvatbaarheidstests en fenotypische analyse, waaronder flowcytometrie, RT-PCR, immunocytochemie en andere cellulaire en moleculaire biologietechnieken.
