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Journal
/
Neuroscience
/
三维运动神经器官生成
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Three-Dimensional Motor Nerve Organoid Generation
Please note that all translations are automatically generated.
Click here for the English version.
三维运动神经器官生成
DOI:
10.3791/61544-v
•
09:57 min
•
September 24, 2020
•
Tatsuya Osaki
2
,
Siu Yu A. Chow
2
,
Yui Nakanishi
2
,
Joel Hernández
3
,
Jiro Kawada
,
Teruo Fujii
,
Yoshiho Ikeuchi
2
1
Institute of Industrial Science
,
The University of Tokyo
,
2
Department of Chemistry and Biotechnology, School of Engineering
,
The University of Tokyo
,
3
Faculty of Science and Engineering
,
Tecnologico de Monterrey
,
4
Jiksak Bioengineering, Inc.
Chapters
00:05
Introduction
00:47
Photolithographic SU-8 Mold Fabrication
01:59
Polydimethlysiloxane (PDMS) Microfluidic-Based Tissue Culture Chip Fabrication
02:43
PDMS Chip Preparation for Motor Nerve Organoid (MNO) Formation
03:40
Induced Pluripotent Stem (iPS) Cell Differentiation
06:39
Motor Nerve Organoid Formation
07:57
Results
09:15
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议提供了一个全面的程序,通过自发组装从组织培养芯片中的球形延伸的强健的轴突束来制造人类iPS细胞衍生的运动神经器官。
Tags
3D Motor Nerve Organoid
IPS Cells
Axon Bundles
Motor Neuron Diseases
SU-8 Photoresist
PDMS Chip
Basement Membrane Matrix
DMEM/F12
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