A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Introduction
- 00:44Quantitative Planarian Behavior Assays
- 02:34Data Analysis
- 05:59Scrunching Induction
- 07:41Results: Species Specific Responses to Near UV Light and Cinnamaldehyde Exposure
- 09:38Conclusion
Freshwater planarians exhibit three gaits (gliding, peristalsis, and scrunching) that are distinguishable by quantitative behavioral analysis. We describe a method to induce scrunching using various noxious stimuli, quantification thereof, and distinction from peristalsis and gliding. Using gene knockdown, we demonstrate the specificity of scrunching as a quantitative phenotypic readout.
